This user guide provides detailed instructions on how to set up, run, and analyze the validation tests, as well as how to troubleshoot common issues. Customize data collection and analysis. Applications. Easy-to-use, robust assay kits for. SpectraMax M series, Gemini, and SpectraMax iDx readers have xenon flash lamps,. SpectraMax Plus 384 reads 96-well plates, 384-well plates, and cuvettes. Download PDF versions of the SpectraMax Microplate Reader User Guides. 384 wells < 45 sec. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. Our microplateSPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentra-tions. In this product video, see how the SpectraMax M5 quickly reads samples. ® reader to the computer. 2 only) SpectraMax M-series Microplate readers. TheSpectraMax®Plus384addstheabilitytoread cuvettes. For more information, please visit us at Devices – Spectramax Gemini XS plate reader (Fluorescence) Molecular Devices – Spectramax 384 plus plate reader (UV/VIS) Invitrogen by Life technologies Qubit 2. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. For more sample throughput, both readers can be easily SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903) Purified luciferase, Promega (Cat# E1701) 384-well solid white plates, Greiner (Cat# 655075) CHO-K1 cells, ATCC (Cat# CCL-61) Complete Growth Media Ham’s F12 Medium, Life Technologies (Cat# 11765-54) SpectraMaxPlus384reads96-wellplates,384-wellplates,andcuvettes. Flexible Throughput Read one sample or up to 384 at a time – it’s your choice. Vice President Sales and Marketing. 0 nm increments Wavelength Bandwidth: 2 nm Wavelength Accuracy: < ± 2. 25 µL/well in a 384-well plate, using AlphaScreen Phosphotyrosine (PT66) Assay Kit 0200-7017. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. Reads 96-well microplates in 5 seconds; Reads 384-well microplates in 16 seconds, Monochromator, tunable 1. Dimensions: 128mm (L) x 86mm (W) x. The optical performance is comparable to a top-of-the-line dedicated Which SpectraMax M Series reader do you need? SpectraMax M Series Multi-Mode Microplate Readers 5 Ordering information VWR cat. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in the plate drawer. Manufacturer: Molecular Devices Model: SpectraMax Will included PC workstation w/ preloaded softmax pro software Wavelength Range: 340 – 850 nm Wavelength Selection: Monochromator, tunable 1. spr * SpectraMax i3(x) SpectraTest ABS2 v1. The optical performance is comparable to a top-of-the-line dedicatedSpectraMax 340PC384的光学设计真实地模 拟双光束分光光度计。每个孔有其各自的 样品光束检测和参比检测。8通道的系统， 包含8条样品检测光束和8条参比光束，给 予出众的精确度和高速读板方式。 不需要滤光片 SpectraMax 340PC384使用光栅单色器代替 滤光片。Explore more about Optimized settings for Transcreener TR-FRET assay on SpectraMax iD5 and SpectraMax i3x readers. The optical performance is comparable to a top-of-the-line dedicatedUnparalleled performance on a personalized platform. craig@californialightworks. 8. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. OurSpectraTest ABS1 User Guide - Molecular DevicesLearn how to use the SpectraTest ABS1 plate to validate the absorbance performance of your microplate reader. The SoftMax® Pro Data Acquisition and Analysis Software controls Molecular Devices® spectrophotometers and absorbance, luminescence, and fluorescence microplate readers andThe absorbance at 450 nm was measured with a microplate reader (SPECTRAmax 384, Molecular Devices, United States). This reader also makes use of a unique temperature. Shaker time 0 to 999 seconds Temperature range Ambient +4°C to 45°C General specifications Dimensions (in. These models can no longer be repaired. The standard 8 filter modes enable endpoint and kinetic applications such as protein quantification, cell viability and ELISA. ester flash assays, in both 96- and 384-well plates. To prevent such interference, select the Interlaced Reading option to read every other well in a checkerboard pattern in the first run and then read the previously omitted wells in a second run. Application note showing how SpectraMax® i3x and SpectraMax®iD5 Multi-Mode Microplate Readers are used to performGq-coupled receptor assays using HTRF technology. ItThe SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. Expand your research capabilities to include flash-based applications, including dual luciferase and ATP assays. Shaker time 0 to 999 seconds Temperature control Ambient. The PathCheck Sensor is a temperature independent feature from Molecular Devices that measures the optical pathlength of samples in microplate wells. Product Specs; Item SpectraMax Plus 384 Spectrophotometer; Company Molecular Devices LLC; This product is no longer available on Biocompare. Page 13: Introduction Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax GEMINI EM. Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine. Stand alone mode is not available for the VersaMax. 100 fmol 384-well (75 µL) 0. 190-1000 nm, tunable in 1 nm increments. Each sample has a discrete sample beam and reference beam. VersaMaxreads96-wellplates. Temperature control and shaking come standard. 2. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). Measurement of tannin, iron-reactive phenolics, anthocyanin and polymeric pigment in red wine is an important part of quality control in the wine industry. Select the Driver tab and click Update Driver. 1. 2 SpectraMax MiniMax300ImagingCytometer(cytometer) 3. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurements in the visible range. microplates or in a cuvette at a selected. Assay Performance with Various Instrument Settings The SpectraMax® M2/M2e instruments use SoftMaxPro® DataSpectraMax® Microplate Readers and SoftMax® Pro Software. The EMax® Plus Microplate Reader is designed to be a versatile and robust plate reader for research laboratories. E = absorptivity (extinction coefficient) C = concentration = pathlength. The SpectraMax. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid-phase, enzyme-mediated reactions (mixing is not critical for liquid-phase reactions). 190-1000 nm. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid-phase, enzyme-mediated reactions (mixing is not critical for liquid-phase reactions). This kit is optimized for Molecular DevicesMolecular Devices Microplate Reader Spectramax 384 340PC. PathCheck Sensor. The SPECTRAmax™ 340PC 384 microplate spectrophotometer provides rapid and sensitive measurements of a variety of analytes across a wide range of concentrations. When connecting for the first time to either the SpectraMax L reader or StakMax system. Detection modalities are shown in Table 1-1. Learn more about its features, specifications, and applications in this user. Light Source: Xenon flash lamp. The . Key features 4 wells: 76 seconds Luminescence Specifications Dynamic Range < 6 decades Sensitivity 10 amol ATP General Specifications. Description Control Panel Cuvette Chamber Back Panel Microplate Drawer The Control Panel TEMP on/off TEMP Figure 1. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. For more information, please visit us at SpectraMax® M2/M2e microplate readers for use with the Transcreener® FI Assays. SoftMax Pro Software, the industry-leading microplate reader software for the SpectraMax Plus 384 system, allows complete customization of data collection and analysis. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid-phase, enzyme-mediated reactions (mixing is not critical for liquid-phase reactions). The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. Detection Cartridge (384) (PN 0200-7015) • SpectraMax Paradigm Glow Luminescence (LUM) Detection Cartridge (96) (PN 0200-7014) A glow luminescence detection cartridge contains the optics and electrical components needed to perform specific measurement modes or for specific applications. 1) All parts of the SPECTRAmax PLUS 384 Microplate Spectrophotometer are warranted for a period of one (1) year from the original date of delivery. Molecular Devices Spectramax Plus 384 Microplate Reader is available from The Lab World Group. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 303 unable to cal dark current. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. SPECTRAmax® PLUS384 is the only spectrophotometer with a built-in cuvette port and microplate drawer. Each sample has a discrete sample beam and reference beam. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The SPECTRAmax™ GEMINI XS Dual-Scanning Microplate Spectrofluorometer. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Beer-Lambert Law states that. 1) All parts of the SPECTRAmax PLUS 384 Microplate Spectrophotometer are warranted for a period of one (1) year from the original date of delivery. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentra-tions. SpectraMax ABS Plus SpectraTest ABS2 v1. 4. GETTING THE MOST FROM YOUR MOLECULAR DEVICES MICROPLATE SYSTEMS applications guide to microplate systems applications guide to microplate systemsSPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentra-tions. Used MOLECULAR DEVICES SPECTRAmax 384 Spectrophotometer For Sale - DOTmed Listing #2356563: Spectra Max Plus 384 Microplate Spectrophotometer - GOOD WORKING UNIT Equipment was recently removed. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. SoftMax Pro. dual monochRomatoRs foR assay flexibility With SpectraMax Multi-Mode Readers, there is no need to utilize expensive filters to optimize detection levels and background. 美谷分子 SpectraMax Plus 384 微孔板读 板机对于进行葡萄酒中苹果酸的酶学定量 检测是很好的一个选择，也可以扩展到类 似的实验，包括氨和挥发性酸度的测定。 这对于花费和时间都是高效的。 SpectraMax Plus 384 微孔板读板机的可 调谐和光径检测传感器特性有助于. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentra-tions. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. If using test tubes, read the absorbance of each tube in the cuvette port of the SPECTRAmax PLUS microplate spectrophotometer. 2) All labor charges to repair the product for a period of one (1) year from the original date of delivery will be paid by Molecular Devices Corporation. Service parts for these models are not being produced and are no longer in stock. Flexible Template Assignment (Figure 1) to customize assays exactly to user specifications. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. UV/Vis spectrophotometer & microplate reader with built-in cuvette port. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. Molecular Devices SpectraMax Plus 384. SpectraMax® M2 and M2e Microplate Readers are the standard for UV/visible multimode reader absorbance, providing ultrafast and full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Multimode Reader Comparison Chart. Fluorescence Intensity top and bottom capabilities. Spectramax i3 manual. Company Molecular Devices LLC. Detection of species collected on membrane plates is also possible. The SPECTRAmax™ 340PC 384 microplate spectrophotometer provides rapid and sensitive measurements of a variety of analytes across a wide range of concentrations. Check Details. 00 USD Tecan Infinite 200 Pro M Plex Microplate Reader - AV $14,995. Temperature control and shaking included as standard. Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays, including. Wavelength(s): 230 to 750 nm (depending on model) Features: Shaking, Temperature Control. The SpectraTest ABS1 plate is a reliable and. The SpectraMax Plus 384 Microplate Reader gives users the convenience of both standard spectrophotometer and microplate reader applications on the same instrument. SpectraMax 340PC384 reads 96-well plates and 384-well plates. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Five-mode microplate reading with superior optics Unique optical characteristics 1. The narrow bandwidths used by the model increase measurement accuracy and linearity over a wide range. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Corning® 384-well Flat Clear Bottom Black Polystyrene TC-treated Microplates, Low Flange, 20 per Bag, with Lid, Sterile Product Number 3764. used. The light transmittance was evaluated as function of wavelength of the BNC membranes. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. The new SPECTRAmax® PLUS 384 is the only spectrophotometer with a built-in cuvette port and microplate drawer. The 190 can read across the entirety of the UV-Vis. The eight-channel system delivers both high precision and speed when reading 96- and 384-well microplates for endpoint measurements, kinetic assays, and spectral scans. In addition, the SpectraMax iD5 The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The SpectraMax L reader is upgradeable to dual-channel configurations for a 2-fold. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeNote: for SpectraMax microplate readers without a cuvette port, the water constant is automatically used in PathCheck calculations, and no Water Constant option appears in the Instrument Settings. With the QuickSync client installed on your computer, your SpectraMax iD3 reader can push data to any computer within the same network. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid-phase, enzyme-mediated reactions (mixing is not critical for liquid-phase reactions). On the front panel of the instrument, press the load/eject button to move the microplate drawer outside of the instrument. Expanded wavelength coverage. 003 fmol 0. 305 reference level saturation. The SpectraMax Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The light should fall square on the slit. This revolution in modularity makes no compromise detection the norm. The optical performance is comparable to a top-of-the-line dedicatedDescription Microplates The SpectraMax Multi-Mode Microplate Reader can accommodate SBS- standard 6-well to 384-well microplates and strip wells. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. For more sample throughput, the system can be. 96, 384 wells Light source Xenon flash lamp (1 joule/flash) Detector Photomultiplier tube (PMT) Read time** 96 wells: 384 wells: Abs 18 sec. Temperature control. , FI 15 sec. SpectraMax® 340PC384 Microplate Reader for acquiring data in endpoint, kinetic, and spectral scan modes using wavelegths from 340-850 nm, tunable in 1 nm increments. Use any standard cuvette, 12 x 75 mm testThe SpectraMax Mini Multi-Mode Microplate Reader is a budget friendly solution. SpectraMax340PC384reads96-wellplatesand384-wellplates. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. . SpectraMax M5. Temperature control and microplate-shaking included. SpectraMax Gemini XPS/EM Register or Sign In. Molecular Devices SpectraMax i3 Multi-Mode Microplate Reader Pred i3x - AV $14,995. The PA 800 Plus system is a robust analytical, kit-based platform that can help you with: Purity/Heterogeneity with gold standard CE-SDS in minutes. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus384 SpectraMax 190 SpectraMax 340PC384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Three low volume 24-well bottom slides. o SpectraMax M2 - Well Scan read mode - single well read coordinate problem fixed. Glad to help you. Instruments with a 96- or 384-well format will accommodate the most commonly used plates. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentra-tions. Upon completion of a plate read, data are delivered to your computer in an Excel-compatible format. The value for 1000 should be higher than the value for 900. Service Contracts, Preventative Maintenance and Performance. 306 plate air cal. Instrument Description and Capabilities: Fluorescence. 100 fmol 1536-well (8 µL) 0. Time Resolved Fluorescence. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Shaker Time: 0 to 999 seconds: Temperature Range. 997 (n = 3). TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross. 384 wells) and the "Select Specific" menu to choose the specific plate type. Act quickly! These items have limited quantity and availability. Molecular Devices also offers a mass spectrometry-based solution for food safety testingThe SPECTRAmax™ GEMINI XS Dual-Scanning Microplate Spectrofluorometer. Key applications**. Molecular Devices Spectramax 384 Plus Microplate Reader is available from The Lab World Group. 2) All labor charges to repair the product for a period of one (1) year from the original date of delivery will be paid by Molecular Devices Corporation. This white, polystyrene ProxiPlate™ has shallow wells allowing for lower volumes to be used in assays. This product is no longer available on Biocompare. Molecular Devices, LLC. Reference diodes enable elimination of measurement noise due to slight fluctuations in. selected when SpectraMax Plus 384 was selected in the Preferences dialog. 315 can't find zero order. SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax™ PLUS microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific pro-teins, nucleic acids, and other molecules across a wide range of concentrations. Molecular Devices Microplate Reader Spectramax 384 340PC. Top detection is available for fluorescence detection on the SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2e. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. Light Source: Xenon flash. Cables. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. An ATP standard curve spanning five decades was run using the ATPlite 1step Luminescence Assay System (PerkinElmer) on the SpectraMax iD3 reader. Manufacturer: Molecular Devices Model: SpectraMax Plus 384 The SpectraMax ® Plus 384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid-phase, enzyme-mediated reactions (mixing is not critical for liquid-phase reactions). The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 1. Shaker time: 0 to 999 seconds Temperature control: Ambient +4°C to 45°C Temperature. Call me at Phoenix Equipment in Rochester. Note: In this user guide, all references to SpectraMax Multi-Mode. The SpectraMax L reader is upgradeable to dual-channel configurations for a 2-fold increase in throughput for medium throughput and secondary screening laboratories. BioRAPTR FRD Microfluidic Workstation and SpectraMax Paradigm Multi-Mode Detection Platform” PART NUMBER: 0200-7017 0200-7018 0200-7019 (384 Std) (384 HTS) (1536 HTS) Plate 96, 384 96, 384 96, 384, formats 1536 Typical 4 min/ 384 2 min/384 8 min/1536 Throughput plate plate plate Fully enabled protocols = reduced setup timeThe SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. SpectraDrop High-Throughput Screening (HTS) Kit. For nearly 30 years, Molecular Devices has provided scientists with . 305 reference level saturation. 3) This warranty covers the SPECTRAmax PLUS 384384-well clear-bottom, scintillating microplate for cell-based radiometric proximity assays. SpectraMax190reads96-wellplates. The following document is intended to demonstrate setup of this. 003 fmol 0. For more sample throughput, the system can be. 0 fluorometer; BioMicroLab – XL20 tube sorter; Biobus; Thermo Scientific – NanoDrop 2000 Spectrophotometer; Thermofisher – Qubit FluorometerSpectraMax® L和SpectraMax® M5酶标仪均为CellTiter-Glo检测提供了优异的检测灵敏度。SpectraMax® L化学发光读板机带有自动增益功能，能同时进行模拟信号和光子计数，提供大于9个数量级的动态范围，对于高信号值样品无需稀释，同时对于低信号样品具有优越的灵敏度。After 3× washing, the plates were developed with ABTS substrate (Moss) and analyzed on a SpectraMax Plus 384 spectrophotometer (Molecular Devices) at optical density (OD) 405 nm. Spectral Range. Simply select the absorbance maximum of a sample and let the monochromator tune to that exact wavelength. Detection modalities are shown in Table 1-1. Other technologies such as imaging. SpectraMax® Plus 384 provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Multimode Reader Comparison Chart. used. Page 6 SpectraMax Plus384, 190, 340PC384, and VersaMax Installation Guide Connecting Instrument Cables The power cord and USB cable connect to the ports on the rear of the instrument. ) 8. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. The SPECTRAmax™ PLUS microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. Details. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. For the reference reading, use the “0” standard. 세포신호전달은 세포가 환경에 반응하고 다른 세포와 소통할 수 있게 합니다…. Molecular Devices Spectramax. 0200-6262. 5 pM 2. VersaMaxreads96-wellplates. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid-phase, enzyme-mediated reactions (mixing is not critical for liquid-phase reactions). Model is Spectramax Plus 384. Also refer to this Multimode Reader Comparison Chart. together with SpectraMax, VersaMax, VMax, and EMax microplate readers from Molecular Devices, offer a quality assurance screening solution for the detection of melamine contamination in such food products as milk, infant formula, pet food, and confectionery. 384-well: Abs 49 sec. Explore how the SpectraMax i3 Multi-Mode Platform provides an unlimited breadth of application possibilities, and how it can evolve with your future needs. Regularly used for NADPH ATPase assays, and BCA assays. It measures the optical density (OD) of samples in both 96- and 384-well. 00 USDThe SpectraMax® M2 and M2e Systems from Molecular Devices are multi-detection microplate readers with dual-. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SPECTRAmax GEMINI XS. For very concentrated samples, use a minimumThe ProcartaPlex 384-well format was specifically developed to increase sample throughput while maintaining the robustness of a 96-well assay. 5 mP 3 mP 1536-well (8 µL) 1. Detection modalities are shown in Table 1-1. ELISAs/EIAs. Tailored to your different needs, these kits are configured and optimized for Molecular Devices SpectraMax® microplate readers with preconfigured protocols provid- The SpectraMax® iD3 Multi Mode Microplate . 2. 3) This warranty covers the SPECTRAmax PLUS 384The SpectraMax 384 Plus UV/Vis Microplate Reader is manufactured by Molecular Devices. 996 (n = 3) ; for SpectraMax Plus 384 reader, r 2 = 0. Review date: 12 Jun 2017 | SpectraMax Plus 384 Microplate Reader Get absorbance measurements from 190 to 1000 nm quickly for samples in test tubes, cuvettes, and 96- or 384-well microplates. SpectraMax ® Plus 384 optics. Transcreener® HTS is a universal, high throughput biochemical assay platform based on the detection of nucleotides, which are formed by thousands of cellular enzymes—many of which catalyze the covalent regulatory. All SpectraMax® M series readers accept cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Contact Us Phone: +1-800-635-5577 Web: Email: [email protected] are some cuvettes that have been tested in the M5 and Plus 384. 1: SpectraMax Plus 384 components. 4% in white 96- and 384-well microplates Sensitivity (ATP-Glow) Optimized 96 wells 3 pM 384 wells 6 pM. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The SpectraMax M4 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96 and 384-well microplates. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. 5014038F 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 11 Applications 12 DualPhotomultiplierTubes 12For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. SpectraMax 190 reads 96-well plates. The SpectraMax Vertical 1000 is available for purchase online and comes with a five-year warranty. Create custom fluorescence assays without having to purchase expensive filter sets. Monochromator, tunable 1. Accessories and spare parts for microplate dispensers, Multidrop 384 and DW. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Eight different automated tests are provided: • Absorbance accuracy (linearity) • Precision (reproducibility) • Stray light • Wavelength accuracy • Wavelength repeatabilityThe SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Now you can run both standard spectrophotometer and microplate reader applications on the same instrument. It is upgradeable to include a barcode reader,. Molecular Devices. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Robust. ability to detect 16 ng/well of DNA and to quantitate 50 ng of . Set up SoftMax Pro Software to use PathCheck as detailed above. Temperature control. View and Download Molecular Devices SPECTRAmax PLUS 384 operator's manual online. 2 5. The SpectraMax 384 Plus lets you get absorbance measurements from 190 to 1000 nm quickly for samples in test tubes, cuvettes, and 96- or 384-well microplates. Wavelength Selection: Monochromator, tunable 1. The control panel consists of a 2-x-20-character LCD and eleven pressure-sensitive membrane keys that can be used to control some functions of the instrument. formats from 6- to 384-wells, the SpectraMax iD3 reader is the complete solution for all your research needs. There should be a value for 900 and 1000. SpectraMax iD3 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. 8 (W) x 15 (D)This SpectraMax Plus 384 Spectrophotometer has been sold, but you can view our other spectrophotometers currently available for purchase here: Plus384, 190, 340PC384, and VersaMax Installation Guide Author: Molecular Devices Subject: Installation Guide Keywords: SpectraMax Versa and others Detection Platform Created Date: 5/17/2023 2:14:27 PMOn SpectraMax L, 1 RLU = 1 count per second for photon counting mode only. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. 0 nm increments Wavelength Bandwidth: 2 nm Wavelength Accuracy: < ± 2. Microplates for applications that require high sample recovery rates. Molecular Devices would like to introduce to you the SoftMax Pro 7, software designed to provide the simplicity, flexibility and power required for advanced. Key features 4 gain calibration failed. Reliable performance with a proven track record. Biocompare is the leading. Statistics. No accessories are needed and SoftMax® Pro Software. Shaker time 0 to 999 seconds Temperature range Ambient +4°C to 45°C General specifications Dimensions (in. and detection of plate formats from 6- to 384-wells, the SpectraMax iD5 reader is the complete solution for all your research needs. General-purpose refrigerators are ideal economical units for when precise temperature control and maintenance are not. 96/384-FortheSpectraMax340PC384,presstoselecttoreada96-wellplateora384-wellplate. The SpectraMax 340PC384 system has a monochromator instead of interference filters. In the M2, M5 and Plus 384, the light. The SpectraMax reader’s optical system mimics a dual-beam spectrophotometer. Detection cartridge modularity allows for easy addition of new read modes within minutes without. . Both the SpectraMax M5 and the Gemini EM are monochromator-based, microplate readers. Abs 49 sec. Such cross talk can occur through the wall of a white 384 well microplate. 384-wells, the SpectraMax iD3 reader is the complete solution for all your research needs. SpectraMax i3x Multi-Mode Microplate Reader. It measures the optical density (OD) of samples in both 96- and 384-well microplates or in a cuvette at a selected wavelength for a single point in time (endpoint), over a specified period of time (kinetic), or over a selected. Select ‘Water Constant’ in the Instrument Settings dialog box (Figure 3). The technology allows automatic adjustment of good absorbance to a tube equivalent path length of 1 cm, converted to 96 or 384 tubes. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Detection of species collected on membrane plates is also possible. Table 2. Multichannel optical design. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. Detection of species collected on membrane plates is also. DNA provides the same sensitivity as a spectrophotometer. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Item SpectraMax 340PC 384 Tunable Spectrophotometer with SoftMax Pro Software; Company Molecular Devices LLC; This product is no longer available on Biocompare. All have an optical pathlength of 1 cm (10 mm) and standard external dimensions (12. 0200-6263. ®Supplier: Molecular Devices. 190-1000 nm, tunable in 1 nm increments. mRNA purity and integrity analysis. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. In case of a cuvette, the optical path is horizontal, hence. Monochromator wavelength selection saves you the time and trouble of ever having to. Features. The SpectraMax M2e are multidetection microplate readers with dual-monochromators, dual-mode cuvette ports, and top- and bottom-reading capability. 313 reference gain check fail. , FI 45 sec. Product Specs. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid-phase, enzyme-mediated reactions (mixing is not critical for liquid-phase reactions). 滤光片型光吸收微孔板读板机（酶标仪），简单易用，性价比高，检测结果精确. SoftMax® Pro Software is included and enables data visualized in 3D plots, gray scale, kinetic curve. Check the optical alignment, you will have to disassemble and remove optical cover. Tb donor/red acceptor. You manually lift up the lid over the chamber to insert or remove a cuvette. One plate carrier adapter is provided with the instrument. Item SpectraMax Plus 384 Microplate Spectrophotometer. Colorimetric assays. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Videos & Webinars. Figure 1. SpectraMax M2e Plate Reader; Application: Absorbance Concentration of Nucleic Acid: Fluorescence Concentration of RNA or DNA: Absorbance, Florescence, Luminescence RNA, DNA, Proteins: Range: 10ng-3000 ng (Accuracy diminishes less than 20 ng/µl and above 1500 ng/µl) RNA Broad Range: 20-1000 ng RNA: 5-100 ng DNA Broad Range: 2. SPECTRAmax® PLUS384 is the only spectrophotometer with a built-in cuvette port and microplate drawer. The SpectraMax L is a dedicated luminescence microplate reader offering outstanding sensitivity, flexibility, automation capability, and validation tools. Read one sample or up to 384 samples in a single plate by using any standard cuvette, or 96- or 384-well microplate. Top detection is available for fluorescence detection on the SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. SpectraMax i3x. For more sample throughput, both readers can be easily integrated into full roboticMode 96-Well 384-Well 1536-Well Optimizedforspeed 20 seconds 33 seconds 68 seconds Optimizedforperformance 28 seconds 45 seconds 96 seconds Stop-and-go(off) 41 seconds 2 minutes <5 minutes.